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目的:本研究探讨了癌睾丸抗原TFDP3与乳腺癌细胞上皮间质化(epithelial-mesenchymal transition,EMT)的关系。方法:本研究中选取了乳腺癌细胞系(MCF-10A,MCF-7,SK-BR-3和MDA-MB-231)作为研究对象,通过Western Blot的方法筛选获得了TFDP3低水平表达的乳腺癌细胞株。进一步通过质粒转染的方式构建TFDP3过表达的细胞系模型,观察TFDP3在EMT中的作用。结果:TFDP3在MCF-10A及SK-BR-3中不表达,在间质化程度较高的MDA-MB-231中高水平表达,而在上皮化程度较高的MCF-7中的低水平表达。MCF-7中过表达TFDP3后,上皮细胞标记分子E-cadherin表达下调,而间质细胞标记分子N-cadherin、Snail、Twist及细胞骨架蛋白Vimentin表达上调。结论:TFDP3可以促进乳腺癌细胞发生EMT。
OBJECTIVE: This study explored the relationship between the cancer testis antigen TFDP3 and the epithelial-mesenchymal transition (EMT) in breast cancer cells. Methods: Breast cancer cell lines (MCF-10A, MCF-7, SK-BR-3 and MDA-MB-231) were selected as the research objects. The low TFDP3 expression in breast was obtained by Western Blot Cancer cell lines. Furthermore, TFDP3 overexpression cell line was constructed by plasmid transfection and the effect of TFDP3 in EMT was observed. RESULTS: TFDP3 was not expressed in MCF-10A and SK-BR-3, but was highly expressed in MDA-MB-231 with a higher degree of interstitial expression but lower in MCF-7 with a higher degree of epithelialization . After over-expression of TFDP3 in MCF-7, the expression of E-cadherin in epithelial cells was down-regulated, while the expressions of N-cadherin, Snail, Twist and Vimentin in the stromal cells were up-regulated. Conclusion: TFDP3 can promote EMT in breast cancer cells.