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筛选肝癌与正常肝基因表达差异克隆。方法应用Ｍｉｃｒｏａｒｒａｙ膜片分别与肝癌和正常肝组织ｍＲＮＡ进行杂交，比较杂交信号，获得表达差异克隆。结果在正常肝中共有９９０个基因表达，其中２５５个较高表达。４２５个ｃＤ┐ＮＡ克隆在正常肝中的表达高于肝癌，其中３３个有较大差异。１９０个ｃＤＮＡ克隆在肝癌中的表达高于正常肝，其中６０个有较大差异。在这些差异克隆中，鉴定出有些是已被他人证实在其它癌中有差异的已知基因。结论应用大规模筛选表达差异的Ｍｉｃｒｏａｒｒａｙ方法，获得了肝与肝癌表达差异的基因克隆。
Screening liver cancer and normal liver gene expression differential clones. Methods Microarray membranes were used to hybridize with mRNA of liver cancer and normal liver tissue respectively. The hybridization signals were compared and differentially expressed clones were obtained. Results A total of 990 genes were expressed in normal liver, of which 255 were highly expressed. The expression of 425 cD┐NA clones in normal liver was higher than that in liver cancer, of which 33 had significant differences. The expression of 190 cDNA clones in HCC was higher than that in normal livers, of which 60 were significantly different. Among these differential clones, some have been identified as known genes that have been confirmed by others to be different in other cancers. Conclusion Using the Microarray method for large-scale screening for differential expression, gene clones with differential liver and liver cancer expression were obtained.