Carbon dots are rapidly emerging carbon-based nanomaterials that,due to their growing applications,will inevitable find their way to natural waters;however,their environmental fate is mostly unknown.Carbon dots with different surface functionality were fabricated and characterized by TEM and FT-IR.Their surface charge,given by the zeta potential,and their hydrodynamic diameter in suspension were investigated under a variety of environmentally relevant conditions.The effect of ionic strength was studied in the presence of monovalent (NaCl) and divalent (CaCl2) cations,for pH levels from 3 to 11;humic acid was used as a model for dissolved natural organic matter.Total potential energies of interactions were modeled by classical DLVO theory.The experimental results showed that water chemistry altered the surface charge of the nanomaterials,but their hydrodynamic size could not be correlated to those changes.Evidence of specific interactions was found for the amino functionalized particles in most cases,as well as the plain carbon dots in the presence of Ca2+ and humic acid.Nanoparticles remained largely stable in suspension,with some exception at the highest ionic strength considered.DLVO theory did not adequately capture the aggregation behavior of the system.Moreover,cation and/or humic acid adsorption negatively affected the emission intensity of the particles,suggesting limitations to their use in naturai water sensing applications.The particular stability shown by the carbon dots results in exposure to organisms in the water column and the possibility of contamination transported to significant distances from their source.