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目的了解沈阳周边山区林区蜱携带新型布尼亚病毒的情况,为沈阳地区发热伴血小板减少综合征的防控提供依据。方法应用蜱种鉴定引物和新型布尼亚病毒特异性引物,采用巢式反转录-聚合酶链反应方法,对2012年4-9月在沈阳周边马耳山、棋盘山和内蒙古与沈阳康平交界地区农户家养动物体表采集的蜱标本1989只进行检测,对扩增得到的目的条带进行基因测序并与GenBank中注册的基因序列进行同源性比较。结果通过对采集的蜱标本进行测序和同源性比较,蜱种鉴定结果为长角血蜱,新型布尼亚病毒检测结果均为阴性。结论目前从沈阳周边山区林区采集的蜱主要为长角血蜱,蜱中尚未发现新型布尼亚病毒的存在,沈阳地区是否存在该病毒的自然疫源地尚有待进一步的深入调查研究,针对新型布尼亚病毒应建立定期监测机制。
Objective To understand the situation of ticks carrying the new Bunyan virus in forest areas around Shenyang, and to provide basis for the prevention and control of fever and thrombocytopenia syndrome in Shenyang area. Methods Using tick species identification primers and new Bunyavirus specific primers, Nested Reverse Transcriptase Polymerase Chain Reaction (PCR) was used to detect the susceptibility of Malus species in the areas of Malcesine, Qipanshan, Inner Mongolia and Kangding, Shenyang from April to September in 2012 The tick specimens collected from the body surface of farmer domestic animals in the junction area were only tested in 1989, and the amplified bands were sequenced and compared with those registered in GenBank. Results The sequencing results and homology comparison of collected ticks showed that the results of tick identification were H. longicornis, and the results of new Bunyavirus were negative. Conclusions At present, the ticks collected from the forest areas around the mountains in Shenyang are mainly H. longicornis. No new Bunia virus has been found in the ticks. Whether there is a natural epidemic area in Shenyang is yet to be further investigated. The new Bunia virus should establish a regular monitoring mechanism.