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基于人血清白蛋白(HSA)在碱性介质中对luminol-H2O2化学发光体系有很强的增敏作用,提出了一种流动注射化学发光测定HSA的新方法。在优化条件下,HSA的线性范围为7.5×10-10～2.8×10-7mol/L,检出限为9.1×10-11mol/L,样品检测频率达102个/h。对2.0×10-8mol/L HSA平行测定11次,RSD为0.9%。方法可应用于实际样品人血清中HSA含量的测定。结合化学发光光谱和紫外可见吸收光谱,对该反应机理进行了探讨。
Based on the strong sensitizing effect of human serum albumin (HSA) on luminol-H2O2 chemiluminescence system in alkaline medium, a new method for the determination of HSA by flow injection chemiluminescence was proposed. Under the optimal conditions, the linear range of HSA was 7.5 × 10-10 ~ 2.8 × 10-7mol / L, the detection limit was 9.1 × 10-11mol / L, and the detection frequency of the sample was 102 cells / h. The parallel determination of 2.0 × 10-8 mol / L HSA 11 times, RSD was 0.9%. The method can be applied to the determination of HSA content in human serum samples. Combined with chemiluminescence and UV-Vis absorption spectra, the reaction mechanism was discussed.