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目的:建立自体髓核移植致大鼠非压迫腰椎间盘髓核突出神经根性疼痛模型,以阿斯匹林为阳性对照药物,运用透射电镜观察方法探讨优化活血利水方对移植髓核、神经根超微结构的影响。方法:40只SD大鼠随机分为对照组、模型组、中药组、阿斯匹林组,建立大鼠自体髓核移植致腰5神经根性痛模型,造模后1周起各组分别予生理盐水1.82mL、生理盐水1.82mL、中药汤剂1.82mL、阿斯匹林1.82mL(0.1g.kg-1.d-1),每日1次,连续用药4周。给药后第3、7天测大鼠左后肢机械刺激痛阈值、第31天取移植髓核和左侧L5神经根作组织病理学电镜观察。结果:术后3、7天模型组、西药组及中药组大鼠左后肢的机械刺激痛阈比术前显著降低。超微结构显示:对照组组织结构正常,模型组明显异常,西药组及中药组显著优于模型组,中药组略优于西药组。结论:优化活血利水方可减轻髓核和神经根病理损害程度。
OBJECTIVE: To establish a rat model of nerve root pain due to non-compression of the lumbar nucleus pulposus induced by autologous nucleus pulposus transplantation, and to use aspirin as a positive control drug. To investigate the effect of Huoxue Lishui Fang on nucleus pulposus and nerve root ultrasonography using transmission electron microscopy. Microstructure effects. METHODS: Forty Sprague-Dawley rats were randomly divided into control group, model group, Chinese medicine group, and aspirin group. The model of lumbar nerve root pain caused by autologous nucleus pulposus transplantation was established. Each group was given one week after the model was established. Normal saline 1.82 mL, physiological saline 1.82 mL, Chinese medicine decoction 1.82 mL, and aspirin 1.82 mL (0.1 g.kg-1.d-1) were administered once a day for 4 consecutive weeks. On the 3rd and 7th day after administration, the left hind limb mechanical stimulation pain threshold was measured. On the 31st day, the transplanted nucleus pulposus and left L5 nerve root were taken for histopathological electron microscopic observation. RESULTS: After 3 and 7 days postoperatively, the mechanical stimulation of the left hindlimb of the model group, the western medicine group and the traditional Chinese medicine group was significantly lower than that before the operation. The ultrastructure showed that the control group had normal tissue structure, the model group was obviously abnormal, the western medicine group and the Chinese medicine group were significantly better than the model group, and the Chinese medicine group was slightly better than the western medicine group. Conclusion: Optimizing Huoxue Lishui can reduce the degree of pathological damage of nucleus pulposus and nerve root.