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利用生物基因组学数据库,对黄瓜NBS类抗病基因类似物(RGA基因)进行生物信息学分析,以预测RGA基因编码产物的理化性质、结构与功能,为进一步研究黄瓜抗病调控机制提供一定的理论依据。结果表明:RGA基因编码产物为一个长度达84个氨基酸的蛋白,其理论分子量为10 964.32Da,理论等电点为PI 9.36,不存在跨膜区域,主要定位在细胞质中;进化比对表明,黄瓜的NBS类抗病基因在亲缘关系上与西瓜最近,其次是丝瓜;二级结构为α-β型,具有较为明显的3个螺旋和2个折叠;具有酰化位点、酪蛋白激酶II磷酸化位点和蛋白激酶C磷酸化位点3个功能位点;预测RGA基因编码产物主要充当辅酶角色参与生化反应的调控,介导基因表达调控,在抗逆相关的信号通路以及种子胚的发育等过程中发挥重要的生理功能。
Bioinformatics analysis was performed on the RBS genes of cucumber NBS with bio-genomics database to predict the physicochemical properties, structure and function of the RGA gene coding products, and to provide some information for further research on the regulation mechanism of Cucumber resistance to disease Theoretical basis. The results showed that the encoded product of RGA was a 84 amino acid protein with a theoretical molecular weight of 10 964.32 Da and a theoretical isoelectric point of PI 9.36. The transmembrane region was not found in the RGA gene and its localization was mainly located in the cytoplasm. The NBS resistance gene of cucumber was phylogenetically related to watermelon, followed by loofah; the secondary structure was α-β type with obvious three helices and two folds; the acylation site, casein kinase II Phosphorylation sites and protein kinase C phosphorylation sites. It is predicted that the encoded product of RGA gene mainly acts as a coenzyme to participate in the regulation of biochemical reactions, and mediates the regulation of gene expression. In the signal pathway related to stress resistance and seed embryo Development and other processes play an important physiological function.