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目的:观察LINGO-1在急性一氧化碳(CO)中毒后大鼠脑白质中的动态表达变化。方法:选择70只SD雄性大鼠随机分为对照组,染毒后1d、3d、7d、14d、21d、28d组(n=10)。采用甲苯胺蓝染色观察染毒后各时间点大鼠脑白质髓鞘脱失情况,并应用免疫组织化学染色及实时定量RT-PCR的方法观察LINGO-1的表达变化。结果:①对照组LINGO-1表达极低,染毒后1d,LINGO-1mRNA和蛋白表达上调均最为显著,3d时下降接近至对照组水平,在7d时均再次出现上调至21d开始下降,28d时LINGO-1mRNA接近对照组水平,而LINGO-1蛋白略高于对照组。②对照组无脑白质脱髓鞘出现,染毒后7d观察到脑白质轴突髓鞘脱失,直至28d脱髓鞘现象仍在发生。结论:在大鼠发生急性CO中毒后,其脑白质中的LNGO-1即发生上调,并呈现二次上调的规律性改变,且二次上调与脑白质脱髓鞘病变发生时间相关联,提示其与CO中毒后脑白质脱髓鞘病变相关。
Objective: To observe the dynamic expression of LINGO-1 in rat white matter after acute carbon monoxide (CO) poisoning. Methods: Seventy male Sprague Dawley rats were randomly divided into control group, n = 10, 1d, 3d, 7d, 14d, 21d and 28d after exposure. Toluidine blue staining was used to observe the demyelination of white matter in rats at each time point. The expression of LINGO-1 was observed by immunohistochemical staining and real-time RT-PCR. Results: ① The expression of LINGO-1 in the control group was extremely low. The mRNA and protein expressions of LINGO-1 in the control group were extremely significantly elevated at 1 day after exposure. The levels of LINGO-1 mRNA and protein were all significantly decreased at 3d, and then decreased to the level of the control group again at 7 days. LINGO-1 mRNA was close to the control group, while LINGO-1 protein was slightly higher than the control group. ② In the control group, there was no demyelination of white matter, and demyelination of white matter axon was observed at 7 days after exposure. Demyelination was still occurring at 28 days. CONCLUSION: After acute CO poisoning in rats, LNGO-1 in the white matter of the rats is up-regulated and shows the secondarily up-regulated regularity, and the second up-regulation is associated with the time of demyelinating lesions It is associated with demyelination of white matter degeneration after CO poisoning.