论文部分内容阅读
目的：研究口腔癌靶向顺铂聚乳酸聚乙二醇纳米粒(CDDP—PLA—PEG—NP,或称隐形顺铂聚乳酸纳米)的急性毒性作用。方法：用不同剂量的CDDP—PLA—PEG—NP和顺铂(CDDP)行小鼠皮下和尾静脉注射,观察小鼠用药后的毒副反应,并计算半数致死量(LD_(50))。结果：CDDP—PLA—PEG—NP皮下和静脉注射的LD_(50)值分别为(28．87±2．02)mg/kg,(15．37±1．23)mg/kg,CDDP皮下和静脉注射的LD_(50)值分别为：(16．82±1．34) mg/kg,(10．62±0．77)mg/kg。结论：CDDP—PLA—PEG—NP对机体的毒副作用无论皮下或静脉注射均小于CDDP,证明CDDP的纳米剂型降低了CDDP药物的毒性作用,为以后的动物实验和临床实验提供了依据。
OBJECTIVE: To investigate the acute toxicity of oral cisplatin-poly (D, L-lactide-co-glycolide) nanoparticles (CDDP-PLA-PEG-NP) Methods: The mice were injected subcutaneously and caudally with CDDP-PLA-PEG-NP and cisplatin (CDDP) at different dosages. The toxicity of the drugs was observed and the LD50 was calculated. Results: The values of LD50 of subcutaneous and intravenous injection of CDDP-PLA-PEG-NP were (28.87 ± 2.02) mg / kg, (15.37 ± 1.23) mg / kg, The LD_ (50) values of intravenous injection were (16.82 ± 1.34) mg / kg and (10.62 ± 0.77) mg / kg, respectively. Conclusion: The toxic and side effects of CDDP-PLA-PEG-NP on the body are less than those of CDDP both in subcutaneous and intravenous injection. It is concluded that the CDDP nano-dosage forms can reduce the toxicity of CDDP drugs and provide the basis for future animal experiments and clinical trials.