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本文报道用吉氏盘尾丝虫非磷酸胆碱抗原的单克隆抗体Og4C3检测班氏丝虫抗原的夹心ELISA法。 Og4C3是吉氏盘尾丝虫雄虫抗原(OgM-Ag)免疫小鼠经融合筛选获得的IgM单克隆抗体,可与吉氏盘尾丝虫、旋盘尾丝虫、犬恶丝虫、犬钩虫和犬弓蛔虫抗原结合,但不与磷酸胆碱抗原决定簇反应。127份个人血清和9份混合血清来自WHO丝虫病血清库。其中包括寄生虫学和/或临床确诊的班氏丝虫病血清68份,马来丝虫病血清10份,帝汶丝虫病血清6份,旋盘尾丝虫病血清28份,罗阿丝虫
This article reports the Sandwich ELISA method for the detection of filariasis Bancroft antigen using the monoclonal antibody Og4C3, a non-phosphocholine antigen of Onchis choriophora. Og4C3 is an IgM monoclonal antibody obtained by fusion screening of O. gondii male mice antigens (OgM-Ag) Hookworms bind to Ascaris canis antigen but do not react with phosphocholine epitopes. 127 personal sera and 9 mixed sera were from the WHO Filariasis Serum Bank. These included 68 parasites and / or clinically diagnosed Bancroftian filariasis sera, 10 Malayan filariasis sera, 6 Serum filaggiosi filariasis, 28 Serian onchocerciasis lines, Filarial