论文部分内容阅读
目的:探讨男性不育症生殖道解脲支原体(UU)感染与细胞因子水平及自身抗体的相关性。方法:按WHO的标准对生育组和不育组(包括少精组、弱精组及无精组)进行精液常规分析,解脲支原体培养;采用酶联免疫吸附实验(ELISA)检测血液和(或)精浆IL-2、IL-6、TNF、AcAb的水平;采用混合抗球蛋白反应试验(MAR)法检测AsAb;采用间接免疫荧光(IFA)法检测ANA。结果:与生育组比较,无精组血液和精浆中IL-2、IL-6、TNF-α水平显著增高,其差异具有统计学意义(P<0.01);弱精症组血液及精浆IL-6水平与生育组相比差异有统计学意义(P<0.05),血液及精浆IL-2、TNF-α水平与生育组相比差异无统计学意义(P>0.05);少精症组血液和精浆中IL-2及血清IL-6与生育组相比差异有统计学意义(P<0.05);在生殖道解脲支原体的培养中不育各组的阳性率均高于生育组;在AsAb、AcAb、ANA的检测中,不育症各组的阳性率均高于对照组,用IFA检测ANA阳性结果中发现,ANA核型均为胞浆颗粒/核颗粒型,而弱精症血清以及不育症各组精浆中未检测出AcAb、ANA。结论:男性生殖道UU感染可诱生细胞因子,打破细胞因子间的平衡,引起体内自身抗体的产生;同时也会影响精液质量,与男性不育密切相关;对男性不育症患者上述指标的检测,并探讨感染、细胞因子及自身抗体之间的相关性,可为不育症的诊断与治疗提供实验依据。
Objective: To investigate the relationship between male infertility genital UU infection and cytokine levels and autoantibodies. Methods: According to the standard of WHO, the spermatozoa were routinely analyzed for reproductive and infertility groups (including oligozoospermia group, weak asthenospermia group and azoospermia group), the culture of Ureaplasma urealyticum was carried out, the levels of blood and serum were determined by enzyme-linked immunosorbent assay (ELISA) Or seminal plasma IL-2, IL-6, TNF, AcAb levels; AsAb by mixed anti-globulin response assay (MAR); ANA by indirect immunofluorescence (IFA) Results: Compared with the fertility group, the levels of IL-2, IL-6 and TNF-α in the serum and seminal plasma were significantly increased in the azoospermia group (P <0.01) There was no significant difference between IL-6 and IL-6 levels in fertility group (P <0.05), IL-2 and TNF-α levels in blood and serum (P> 0.05) The positive rates of IL-2 and IL-6 in serum and seminal plasma were significantly different from those in reproductive group (P <0.05). The positive rates of genital Ureaplasma Fertility group; in the detection of AsAb, AcAb, ANA, the positive rate of infertility groups were higher than the control group, IFA test positive for ANA found that ANA karyotype are cytoplasmic granules / nuclear particle type, and AcAb and ANA were not detected in the seminal plasma of asthenospermia and infertility groups. Conclusion: UU infection of male genital tract can induce cytokines, break the balance between cytokines and cause the production of autoantibodies in the body, but also affect the quality of semen, which is closely related to male infertility. In the male infertility patients, Detection, and to explore the correlation between infection, cytokines and autoantibodies, can provide experimental evidence for the diagnosis and treatment of infertility.