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Objective:To investigate the effect of Radix Isatidis polysaccharides(RIP)on the immunological function and expression of immune related cytokines in mice.Methods:Sixty mice were randomly divided into six groups,the normal group,cyclophosphamide(Cy)model group,Levamisole (positive control)group,RIP low,medium and high dose groups(0.08 g/kg,0.16 g/kg,0.32 g/kg, respectively),ten in each group.By detecting the value of abdominal macrophage phagocytic index, serum hemolysin(HC_(50)),proliferation of lymphocytes and expression of related cytokines,interleukin(IL-2) and interferonγ(INF-γ),the effect of RIP on immunological function and its mechanism were studied. Results:RIP could improve the level of hemolysin in immunological function depressed mice.The results showed that the value of macrophage phagocytic index in the high dose RIP group increased from 1.11±0.13 to 1.42±0.26.The level of IL-2 and INF-γcould be decreased by Cy to 38.12±6.88 ng/L and 139.23±29.87 ng/L,respectively,while RIP in high dose could increase the secretion of IL-2 and INF-γ, to 53.54±14.43 ng/L and 189.91±32.63 ng/L,respectively.Conclusion:RIP could enhance non-specific immunological function,humoral immunity and cellular immunity in mice.
Objective:To investigate the effect of Radix Isatidis polysaccharides(RIP) on the immunological function and expression of immune related cytokines in mice.Methods:Sixty mice were randomly divided into six groups, the normal group,cyclophosphamide(Cy)model group,Levamisole ( Positive control)group,RIP low,medium and high dose groups(0.08 g/kg,0.16 g/kg,0.32 g/kg, respectively),ten in each group.By detecting the value of abdominal macrophage phagocytic index, serum hemolysin( HC_(50)), proliferation of lymphocytes and expression of related cytokines, interleukin(IL-2) and interferonγ(INF-γ), the effect of RIP on immunological function and its mechanism were studied. Results:RIP could improve the level of Hemolysin in immunological function raised mice.The results showed that the value of macrophage phagocytic index in the high dose RIP group increased from 1.11±0.13 to 1.42±0.26.The level of IL-2 and INF-γcould be decreased by Cy to 38.12± 6.88 ng/L and 139.23±29.87 ng/L, respectively, While RIP in high dose could increase the increase of IL-2 and INF-γ, to 53.54±14.43 ng/L and 189.91±32.63 ng/L,respectively.Conclusion:RIP could enhance non-specific immunological function,humoral immunity and cellular Immunity in mice.