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本研究应用~(125)I-UdR释放实验,测定了大量随机取样于111位癌症患者的外周血淋巴细胞以及从它们中诱导出的处于不同培养期的淋巴因子激活的杀伤细胞对K562和Raji肿瘤细胞的体外细胞毒活性.对560个有效数据的统计分析,获得以下结果:1.经过培养系统的作用,对K562细胞的细胞毒活性,从培养前的34.78±25%上升到8～13天培养期的68.04±17.3%之后基本稳定于近70%的水平,直至23～27天的培养期.此细胞毒活性的构成比模式表明,在培养之前,样品分布于很广的活性区域(10～90%);培养 8～13天和以后的培养期,约85%的样品集中到高活性区域(50～95%).2.对 Raji细胞的细胞毒活性,从培养前的8.9±8%上升到8～13天培养期的42.1±22%之后,在随后的培养期中,维持于约35%的水平.对Raji的细胞毒活性的构成比显示,在培养前,全部样品处于低活性区域(<25%);在培养过程中,部分样品(约30%)向高活性区域发生明显的扩展(50～90%),但另一部份样品(约40%)一直位于低活性区域(<35%).这样形成高低两个分布区域.这种现象的机理值得进一步探讨.
In this study, a ~125 I-UdR release assay was used to determine a large number of peripheral blood lymphocytes randomly sampled from 111 cancer patients and the lymphokine-activated killer cells induced in them from different culture phases to K562 and Raji. In vitro cytotoxic activity of tumor cells. Statistical analyses of 560 valid data yielded the following results: 1. The cytotoxic activity of K562 cells upon culture system increased from 34.78±25% before culture to 8～13. After 68.04±17.3% of the culture period, it was almost stable at the level of nearly 70% until the culture period of 23 to 27 days. This pattern of cytotoxic activity ratios indicates that the samples were distributed in a wide range of active areas before culture. 10 to 90%); culture 8 to 13 days and later culture period, about 85% of samples concentrate to high activity areas (50 to 95%). 2. Cytotoxic activity to Raji cells from 8.9± before culture 8% rises to 42.1±22% of the 8- to 13-day culture period, and is maintained at about 35% in the subsequent culture period. The composition ratio of cytotoxic activity to Raji shows that all samples were low before culture. Active area (<25%); During the culture process, some samples (about 30%) go to the high activity area Obvious extension (50-90%), but another part of the sample (about 40%) has been active in the low region (<35%). Such low formed two distribution areas. Mechanism of this phenomenon is worthy of further investigation.