【摘 要】
：
目的 评价人类脂肪来源成体干细胞(hADASc)体外培养转基因定向成软骨诱导的可行性.方法 hADASc体外培养后行PGL3.转化生长因子β1(TGF-β1)基因转染并鉴定,检测转染细胞Lueif
论文部分内容阅读
目的 评价人类脂肪来源成体干细胞(hADASc)体外培养转基因定向成软骨诱导的可行性.方法 hADASc体外培养后行PGL3.转化生长因子β1(TGF-β1)基因转染并鉴定,检测转染细胞Lueiferase活性相对光读数(RLU),再行反转录聚合酶链式反应(RT-PCR)检测和抗Ⅱ型胶原免疫组织化学染色(实验组:转染的hADASc,同比对照组:加入软骨细胞定向诱导培养基的未转染hADASc;阴性对照组:未转染的hADASc),自动图像分析系统分析免疫染色图片并计算阳性颗粒(PU)值.结果 人类皮下脂肪体外分离培养出具有干细胞特征的有核组织细胞.hADASc转染后,实验组测得RLU值(9 212.583±315.240)高于阴性对照组(317.000±20.710,P＜0.01).RT-PCR结果显示转染的hADASc表达TGF-β1;免疫染色结果显示,实验组与同比对照组呈阳性反应,且两组PU值(13.864±2.416,13.637±2.548)均与阴性对照组的PU值差异有统计学意义(6.013±0.827,P＜0.05).结论 人类皮下脂肪体外分离培养出具有干细胞特征的有核组织细胞.PGL3-TGF-β1基因转染的hADASc可表达TGF-β1.内源性TGF-β1可诱导hADASc分泌Ⅱ型胶原蛋白,与外源性TGF-β1作用相类似.
其他文献
Up-scaled deposition process of Teer-UDP850/4 has been established and used for massive production of CrTiAlN hard coatings in applications of anti-wear, cuttin
Phase separation process of Ni42CrTiAl alloy aged at various temperatures was studied by means of transmission electron microscope and X-ray diffraction. It was
The oxidation behavior of high speed steel (HSS) was researched by high temperature thermo balance at 500to 800℃ in water vapor. The morphology was observed by
A system was developed to identify characteristics of quenchants. It consists of computerized hardware,temperature acquisition software as well as changeable pr
To minimize quenching distortion and dispersion, carburizing and quenching process conditions must be optimized; this includes the parts racking design used for
目的评价B超引导下经皮肾镜碎石取石术治疗复杂性肾结石的疗效及安全性。方法收集并分析2005～2012年586例完成该手术的复杂性肾结石患者资料。结果手术均取得成功,一期结石取
Objective: To explore the feasibility of the full automatic animal experimental cabin to establish the animal models in normobaric/hypobaric hypoxic and high ca
在此根据玉米在不同生长阶段对养分的吸收,阐述了在不同生产阶段所适用的肥料及玉米的病虫害的防治。
Based on the absorption of nutrients in different growth stages o
在4990m2水稻田内进行了水稻、河蟹生态立体种养殖技术试验,试验采用在稻田开挖环沟、建设河蟹防逃设施,采用“大垄双行、边行加密”的水稻插播技术,经过5个月的生产试验,试
近些年来,农信社的改革与发展取得了长足的进步,但由于产权关系的扭曲和经营机制的不规范,农村信用社的发展受到了严重的阻碍.本文以江西省进贤县农村信用社改革为例,探索其