大豆疫霉根腐病是危害大豆生产的毁灭性病害之一,研究利用抑制性消减杂交技术(Suppression Subtrac-tive Hybridization,SSH)成功构建了疫霉菌诱导的大豆抗病品种绥农10差异表达的cDNA消减文库,从文库中共筛选到2 067个阳性克隆,PCR鉴定插入片段大部分集中在100~800 bp之间,利用BLAST在GenBank数据库进行序列相似性比对,获得有功能的EST375个,分析表明这些EST功能涉及大豆的抑制病原菌生长、细胞自身保护、信号传导、系统获得抗性、蛋白质合成、呼吸作用等。 Phytophthora sojae is one of the devastating diseases that endanger the production of soybean. We successfully constructed the differential expression of Suinong 10 induced by Phytophthora infestans using Suppression Subtrac-tive Hybridization (SSH) cDNA subtracted libraries. A total of 2 067 positive clones were screened from the library. Most of the inserted fragments were identified from 100 to 800 bp. BLAST was used to perform sequence similarity analysis in the GenBank database to obtain 753 ESTs with functional analysis. It is indicated that these EST functions involve the inhibition of soybean growth by pathogen, cell self-protection, signal transduction, system resistance, protein synthesis, respiration and the like.