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灭多威对环境中非靶标生物的毒性作用广受关注。选用果蝇胚胎S2细胞和人肝癌HepG2细胞为研究对象,采用噻唑蓝(MTT)细胞活力测定、单细胞彗星电泳和磷酸化组蛋白(γH2AX)免疫荧光印迹等方法研究了灭多威的细胞毒性。结果表明:随着灭多威浓度的升高,S2和HepG2细胞活力均呈逐渐下降趋势;两种细胞均出现显著的彗星现象,且彗星尾长增加,尾部面积增大;γH2AX阳性细胞百分率逐渐增大。表明果蝇S2和人HepG2细胞DNA受损程度随着灭多威剂量的增大而加重,灭多威可诱导细胞DNA双链断裂,最终导致细胞凋亡。灭多威具有潜在的基因毒性,长时间接触会损害人类健康。
Methomyl on the non-target organisms in the toxic effects of widespread concern. The Drosophila embryonic S2 cells and human hepatocellular carcinoma HepG2 cells were selected as research objects. MTT assay, single cell comet assay and phosphorylated histone (γH2AX) immunofluorescence were used to study the cytotoxicity of methomyl . The results showed that the viability of S2 and HepG2 cells decreased gradually with the increase of metoclopiride concentration. Both of them showed significant comet phenomenon, and the tail length increased and the tail area increased. The percentages of γH2AX positive cells gradually increased Increase. The results showed that DNA damage in Drosophila S2 and human HepG2 cells was aggravated with the increase of the dosage of methomyl, and methomyl could induce DNA double-strand breaks in cells and eventually lead to apoptosis. Methomyl has the potential genotoxicity, prolonged exposure can damage human health.