本文介绍了一种从水肿胎儿的羊水样本中直接检测B_(19)细小病毒壳体抗原的简单快捷的产前诊断方法。用抗两种壳体蛋白VP_1和VP_2的单克隆抗体和过氧化物酶标记的第二抗体揭示羊水中B_(19)抗原的存在。VP_1和VP_2分别代表着4％和96％的B_(19)壳体,其结果与用杂交技术和巢式聚合酶链反应(PCR)获得的结果进行比较。本文选择26例非免疫性胎儿水肿用斑点杂交、原位杂交和巢式PCR三种方法至少有一种在羊水中测到B_(19)DNA即诊为胎儿B_(19)感染,其中13 This article presents a simple and rapid prenatal diagnosis method for the direct detection of B 19 parvovirus antigen in the amniotic fluid samples from edematous fetuses. The presence of B 19 antigens in amniotic fluid was revealed by monoclonal antibodies against both capsid proteins VP_1 and VP_2 and secondary antibodies labeled with peroxidase. VP_1 and VP_2 represent 4% and 96% B_ (19) shell, respectively. The results were compared with the results obtained by hybridization and nested polymerase chain reaction (PCR). In this paper, 26 cases of non-immune fetal edema were detected by FISH, in situ hybridization and nested PCR at least one of which was diagnosed as B 19 infection in amniotic fluid, of which 13