论文部分内容阅读
通过反射干涉对比显微技术和快速图像分析技术,捕获细胞质膜外光晕变化和细胞周边边缘激发变化的信息,并采用闪烁本征模式分解测定柠檬醛作用于黄曲霉质膜前后各模式（m=0,1,2）所对应的平均平方振幅<δn2m>、剪切弹性μ和弹性弯曲模量Kc,与理论模式光谱相比,研究柠檬醛损伤质膜的物理机制.结果表明,m=1的平动模式主导了光晕波谱,其平均平方振幅呈双指数衰变,该模式几乎与剪切弹性无关,即μ=0;弹性弯曲模量Kc的最小和最大值分别为9.45×10-18J和5.43×10-17J,与柠檬醛的浓度成正相关性.揭示该醛对黄曲霉质膜的损伤,除改变膜的选择通透性和电导率外,同时使质膜平动模式的平均平方振幅呈双指数衰变,弹性弯曲模量Kc增加,从而降低膜伸缩的弹性.
The information about changes in the extracellular plasma membrane luminescence and the peripheral edge of the cell periphery was captured by the contrast interference microscopy and rapid image analysis. The scintillation eigenmodel decomposition was used to determine the effects of citral on the flavonoids of Aspergillus flavus = 0,1,2) corresponding to the average square amplitude <δn2m>, shear elasticity μ and elastic bending modulus Kc, compared with the theoretical model spectrum to study the citral damage plasma membrane physical mechanism.The results show that m = 1 translational mode dominates the halo spectrum, and its average square amplitude shows double exponential decay, which is almost independent of the shear elasticity, that is μ = 0; the minimum and maximum values of elastic bending modulus Kc are 9.45 × 10- 18J and 5.43 × 10-17J, and the concentration of citral has a positive correlation.The disclosure of the aldehydes on the Aspergillus flavus membrane damage, in addition to changing the membrane permeability and conductivity, while the average membrane translational mode of the average Squared amplitude showed double exponential decay, elastic modulus of elasticity Kc increased, thereby reducing the flexibility of membrane stretching.