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以垂枝桦(Betula pendula Roth.)具节茎段为外植体,对其组培快繁体系进行了比较系统的研究。结果表明,将垂枝桦茎段外植体在MS+6-BA 0.5 mg/L+KT 1.25 mg/L+NAA 0.1 mg/L+3%Sucrose+0.7%Agar(p H 5.8)培养基上培养,不定芽萌发率最高;将其不定芽在MS+6-BA 0.75 mg/L+KT 0.75 mg/L+NAA 0.1 mg/L+3%Sucrose+0.7%Agar(p H 5.8)培养基上继代培养一个月左右,不定芽继代增殖系数可达到5.0~7.0;垂枝桦试管苗在1/2MS+KT 0.05 mg/L+NAA 0.3 mg/L+1.5%Sucrose+0.7%Agar(p H 5.8)培养基上,生根率为96.7%;经驯化、移栽后,垂枝桦生根试管苗成活率可达到90.0%以上。
In this study, Betula pendula Roth. Was used as explants to conduct a systematic study on its tissue culture system. The results showed that the stem explants of Betula platyphylla were cultured in MS + 6-BA 0.5 mg / L + KT 1.25 mg / L + NAA 0.1 mg / L + 3% Sucrose + 0.7% Agar The adventitious buds had the highest rate of germination. The adventitious buds were subcultured on MS + 6-BA 0.75 mg / L + KT 0.75 mg / L + NAA 0.1 mg / L + 3% Sucrose + 0.7% Agar After subculture for one month, the multiplication coefficient of adventitious buds reached 5.0-7.0. The shoot density of shoots of Betula platyphylla in the medium of 1 / 2MS + KT 0.05 mg / L + NAA 0.3 mg / L + 1.5% Sucrose + 0.7% Agar ) Medium, the rooting rate was 96.7%. After acclimation and transplanting, the survival rate of Betula platyphylla rooted in vitro could reach 90.0%.