论文部分内容阅读
目的 :观察survivin蛋白在氟中毒SD大鼠牙胚发育过程中的表达,探讨氟对survivin蛋白表达的影响,进一步探讨氟斑牙的发病机制。方法:取妊娠10 d孕鼠45只,随机分为对照组、实验1组和实验2组,饮水中含氟浓度分别为0、50、150 mg/L。每组分别在E18.5 d(妊娠18.5 d)、E20.5 d、P1.5 d(出生后1.5 d)、P3.5 d、P5.5 d时取材、固定、包埋,选择P1.5 d标本做H-E染色。应用免疫组织化学SABC法对每组切片染色。利用Motic Med 6.0数码医学图像分析系统(A)进行图像采集。采用SPSS 13.0软件包对数据进行单因素方差分析。结果:在牙胚发育的不同时期,各组survivin蛋白的表达均呈“M”形变化趋势。E18.5d组F=1.050,P>0.05;E20.5 d组F=2.232,P>0.05,各组间差异无显著性;P1.5 d组F=3.538,P<0.05,P3.5 d组F=3.820,P<0.05,P5.5 d组F=5.096,P<0.05。实验组与对照组survivin的表达有显著差异。新生鼠各组经SNK法比较,对照组与实验1组之间的survivin表达差异无显著性(P>0.05);对照组与实验2组之间的差异有显著性(P<0.05)。结论:氟使新生鼠survivin蛋白表达量减少,且氟浓度越高,这种抑制作用越明显,可能是氟牙症发生的一条途径。
OBJECTIVE: To observe the expression of survivin protein during tooth germ development of fluorosis SD rats and to explore the effect of fluoride on the expression of survivin protein, further explore the pathogenesis of dental fluorosis. Methods: Forty-five pregnant rats of 10 d pregnancy were randomly divided into control group, experiment 1 group and experiment 2 group. The concentrations of fluoride in drinking water were 0, 50 and 150 mg / L respectively. Each group was taken on E18.5 d (18.5 d of gestation), E20.5 d, P1.5 d (1.5 d after birth), P3.5 d, and P5.5 d, fixed, embedded and selected P1. 5 d specimens for HE staining. Each group of sections was stained with immunohistochemical SABC method. Using Motic Med 6.0 digital medical image analysis system (A) for image acquisition. Data were subjected to one-way analysis of variance using the SPSS 13.0 software package. Results: At different stages of tooth germ development, the expression of survivin protein showed “M” shape change trend. F = 1.050, P> 0.05 for E18.5d group; F = 2.232, P> 0.05 for E20.5d group; there was no significant difference between groups Group F = 3.820, P <0.05, P5.5 d group F = 5.096, P <0.05. The expression of survivin in experimental group and control group were significantly different. There was no significant difference in survivin expression between control group and experimental group 1 (P> 0.05). There was significant difference between control group and experimental group 2 (P <0.05). CONCLUSION: Fluorine decreases the expression of survivin protein in neonatal rats, and the higher the fluoride concentration, the more obvious this inhibitory effect may be a way of occurrence of dental fluorosis.