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目的:比较柔红霉素(DNR)、羟基脲(HU)、高三尖杉酯碱(HHT)对三氧化二砷(ATO)诱导的急性早幼粒细胞白血病(acute promyelocytic leukemia,APL)细胞促凝血活性的影响。方法:应用0.25μmol·L-1ATO处理NB4细胞6 d,采集入院时无白细胞增高、ATO治疗后出现高白细胞血症(WBC≥10×109L-1)的6例APL患者外周血,分离出单个核细胞(APL细胞)。在体外用治疗浓度的DNR、HU、HHT处理NB4/APL细胞24 h,检测细胞凋亡率及促凝血活性。结果:HU和DNR可增强NB4细胞促凝血活性(P<0.05),而HHT处理后其促凝血活性不增强(P>0.05);APL细胞在体外经HHT处理,可显著增加细胞凋亡比例(P<0.05),且促凝血活性无明显增强(P>0.05),而DNR和HU在促进细胞凋亡的同时,也增强了促凝血活性(P<0.05)。结论:DNR、HHT、HU都可以诱导ATO处理的NB4/APL细胞凋亡,其中以HHT促凋亡作用最强,促凝血活性影响最小。
Objective: To compare the effects of DNR, HU and HHT on the procoagulant activity of ATO-induced acute promyelocytic leukemia (APL) cells influences. Methods: NB4 cells were treated with 0.25μmol·L-1 of ATO for 6 days. No leukocytosis was found on admission. Peripheral blood was collected from 6 APL patients with hyperlipidemia (WBC≥10 × 109L-1) after ATO treatment. A single Nuclear cells (APL cells). NB4 / APL cells were treated with DNR, HU and HHT at a therapeutic concentration in vitro for 24 h, and the apoptosis rate and procoagulant activity were measured. Results: HU and DNR enhanced the procoagulant activity of NB4 cells (P <0.05), while the activity of procoagulant was not enhanced after HHT treatment (P> 0.05). The HUVECs treated with HHT significantly increased the percentage of apoptotic cells (P <0.05), and the procoagulant activity was not significantly increased (P> 0.05). However, DNR and HU enhanced the procoagulant activity (P <0.05). CONCLUSION: DNR, HHT and HU can all induce the apoptosis of NB4 / APL cells treated with ATO. Among them, the effect of HHT on apoptosis is the strongest and the procoagulant activity is the least.