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将超光谱成像技术与显微镜技术相结合,研制了推帚式显微超光谱成像仪.使用该设备采集了正常、糖尿病和药物治疗大鼠的视网膜组织切片的显微超光谱图像数据.通过对正常对照组、糖尿病组、药物治疗组共40例样本的显微超光谱数据进行处理,获得了3组样本的单波段图像和三波段伪彩色合成图像,并提取了各组样本外核丛的典型透射率光谱曲线.分析这些曲线发现,糖尿病大鼠视网膜外核丛组织细胞在400~800nm光谱范围内的透射率整体高于正常大鼠,并且在第180波段附近出现一个小的吸收峰,注射LCVS1001药物治疗后大鼠视网膜外核丛组织的透射率介于正常组与糖尿病组之间,且180波段附近的吸收峰变弱,因此该药物对大鼠糖网病有一定的治疗作用.通过对3组样本的图像和光谱特征的分析表明,可以将显微超光谱成像仪作为一种新的手段,辅助医学研究人员对糖网病的发病和致盲机制以及各种药物的治疗效果进行研究.
Combining hyperspectral imaging with microscopy, a push-broom microscopic hyperspectral imager was developed, using this device to acquire microscopic hyperspectral image data of retinal tissue sections from normal, diabetic, and drug-treated rats. 40 samples of normal control group, diabetic group and drug treatment group were processed by microscopic hyperspectral data to obtain three groups of single-band images and three-band pseudo-color composite images, Typical transmittance spectral curves. Analysis of these curves showed that the transmissivity of extracellular perinuclear plexus cells in the 400-800 nm range of diabetic rats was generally higher than that of normal rats, and a small absorption peak appeared around the 180th band. After injection of LCVS1001, the trans retinal extracellular nuclear plexus tissue transmission between the normal group and the diabetic group, and the absorption band around 180 weakened, so the drug has a certain therapeutic effect on diabetic nephropathy. Through the analysis of the images and spectral characteristics of the three groups of samples, it is indicated that the microscopic hyperspectral imager can be used as a new method to assist the medical researchers in the research on sugar network Pathogenesis and treatment of blindness and various mechanisms of drug research.