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目的:研究血栓心脉宁片对血管性痴呆(VD)模型大鼠的保护作用。方法:通过双侧颈总动脉反复夹闭再灌注同时ip给予硝普钠(2.5 mg/kg)以复制大鼠VD模型。实验分为正常对照[等容0.5%羧甲基纤维素钠(CMC-Na)溶液]组、模型(等容0.5%CMC-Na溶液)组、吡拉西坦(0.5 g/kg)组与血栓心脉宁高、低剂量(2.2、1.1 g/kg)组,ig给药,每天1次,连续2个月。水迷宫法测定学习期(前6 d)大鼠潜伏期、游程,测试期(第7天)大鼠穿台次数、穿过有效区次数、平台停留距离、平台停留时间、有效区停留距离、有效区停留时间及相应比值。制作大鼠脑部切片并通过显微镜进行大鼠海马与皮质病理形态学观察。结果:在学习期,与正常对照组比较,模型组第1、2、3、4、6天大鼠潜伏期延长、游程增加(P<0.01或P<0.05);与模型组比较,血栓心脉宁高、低剂量组每时间点潜伏期与游程均有一定缩短(P<0.01或P<0.05)。在测试期,与正常对照组比较,模型组除有效区停留距离、有效区停留距离/总路程之比、有效区停留时间/总时间之比以外,其余指标均减少(P<0.01或P<0.05);模型组大鼠皮质、海马神经细胞数减少,噬神经现象较多。与模型组比较,血栓心脉宁高、低剂量组各指标均有一定改善(P<0.01或P<0.05)。结论:血栓心脉宁对VD大鼠学习记忆能力具有改善作用,能提高其定位航行和空间探索能力,改善受损海马及皮质组织。
Objective: To study the protective effect of XHXM on vascular dementia (VD) model rats. Methods: Rat models of VD were duplicated by repeated occlusion of bilateral common carotid arteries and reperfusion with sodium nitroprusside (2.5 mg / kg). The experiment was divided into normal control group (0.5% CMC-Na solution) and isofluracetam (0.5 g / kg) Thromboembolism Ning high, low dose (2.2,1.1 g / kg) group, ig administration, once a day for 2 months. Water labyrinth method was used to determine the latency, run length and run time of rat during the study period (first 6 days), the number of wearing trains, the number of passing through the effective area, the stopping distance of the platform, the dwell time of the platform and the stopping distance of the effective area District residence time and the corresponding ratio. The rat brain sections were made and the histopathology of hippocampus and cortex was observed by microscope. Results: During the study period, compared with the normal control group, the latency of rats in the model group was prolonged and the run length increased on the 1st, 2nd, 3rd, 4th and 6th days (P <0.01 or P <0.05). Compared with the model group, The latency and run length at each time point in Ninggao high and low dose groups were both shortened (P <0.01 or P <0.05). During the test period, compared with the normal control group, the other indexes in the model group except for the effective area stay distance, effective area stay distance / total distance ratio, effective area stay / total time ratio decreased (P <0.01 or P < 0.05). In the model group, the number of cortical and hippocampal neurons decreased and the phenomenon of autophagy was more. Compared with the model group, each index of thrombus and heart pulse in high and low dose groups had a certain improvement (P <0.01 or P <0.05). Conclusion: XJXD can improve the ability of learning and memory of VD rats, improve the capability of positioning navigation and space exploration, and improve the damaged hippocampus and cortical tissues.