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目的比较两种脑出血(ICH)动物模型磁共振成像(MRI)的差异,并对其分子机制进行探讨。方法将胶原酶和自体血注入大鼠尾状核建立脑出血模型,分别于术后6h、12h、1d、2d、3d和7d进行磁共振成像扫描,观察两组脑出血模型血肿体积的大小;运用干湿重法、伊文思蓝(EB)测定法和免疫印迹分别检测两组脑出血模型的脑含水量(BWC)、血脑屏障(BBB)通透性和AQP4的表达变化。结果胶原酶模型组的血肿体积在脑出血后1d达到最大并持续至3d,自体血组的血肿体积在12h达到最大;胶原酶模型组的BWC在1d达到高峰并持续至3d,而自体血组BWC在2d逐渐降低;胶原酶模型组的EB含量在12h达到高峰并持续至2d,而自体血组EB含量在1d逐渐下降;两组模型的AQP4表达量在6h开始升高,1d达到高峰,2d逐渐降低。两组模型比较具有统计学差异(P<0.05)。结论胶原酶对BBB和细胞外基质的破坏以及AQP4的表达变化是两组脑出血模型血肿体积出现差异的重要原因。
Objective To compare the difference of magnetic resonance imaging (MRI) in two animal models of intracerebral hemorrhage (ICH) and to explore its molecular mechanism. Methods The model of intracerebral hemorrhage was established by injecting collagenase and autologous blood into the caudate nucleus of rats. The rabbits were subjected to magnetic resonance imaging scanning at 6h, 12h, 1d, 2d, 3d and 7d respectively to observe the hematoma volume of the two groups. The changes of brain water content (BWC), BBB permeability and AQP4 expression in cerebral hemorrhage model were detected by dry-wet method, Evans blue (EB) assay and Western blot respectively. Results The volume of hematoma in collagenase model group reached the peak at 1 day after cerebral hemorrhage and lasted for 3 days. The volume of hematoma in autologous blood reached its maximum at 12 hours. The BWC in collagenase model group peaked at 1 day and continued to 3d, BWC decreased gradually at 2d; the EB content in collagenase model group peaked at 12h and continued to 2d, while EB content in autologous blood group decreased gradually at 1d. The expression of AQP4 in both groups increased at 6h and peaked at 1d, 2d gradually decreased. The two groups had statistically significant difference (P <0.05). Conclusion The destruction of BBB and extracellular matrix and the change of AQP4 expression by collagenase are the important reasons for the difference of hematoma volume between the two groups.