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目的探讨低氘水(deuterium-depleted water,DDW)对肝癌HepG2细胞增殖、侵袭能力的抑制作用。方法 HepG2细胞及正常肝细胞LO2用50、75、100、150ppm DDW(纯净水对照组)处理,用MTT法、平皿克隆实验检测细胞增殖、侵袭转移能力,Western blot检测增殖细胞核抗原(PCNA)表达。结果 100ppm DDW处理24h对LO2细胞、72h对HepG2增殖抑制无影响(P>0.05)。HepG2在50、75ppm DDW中细胞克隆数均低于对照组,而LO2细胞克隆数高于对照组(P<0.01)。50、75、100ppm DDW处理后HepG2细胞PCNA表达明显低于对照组(P<0.01)。结论 DDW可抑制HepG2细胞增殖和侵袭能力,但促进正常细胞LO2生长,这可能与PCNA表达下调有关。
Objective To investigate the inhibitory effect of deuterium-depleted water (DDW) on the proliferation and invasion of HepG2 cells. Methods HepG2 cells and normal liver cells (LO2) were treated with 50, 75, 100 and 150 ppm DDW (pure water control group) respectively. Cell proliferation, invasion and metastasis were detected by MTT assay and plate cloning assay. PCNA expression was detected by Western blot . Results 100ppm DDW treatment 24h LO2 cells, 72h HepG2 proliferation had no effect (P> 0.05). The number of clones of HepG2 in 50,75 ppm DDW was lower than that in control group, while the number of LO2 cells was higher than that in control group (P <0.01). The PCNA expression in HepG2 cells treated with 50,75,100 ppm DDW was significantly lower than that in the control group (P <0.01). Conclusion DDW can inhibit the proliferation and invasion of HepG2 cells, but promote the growth of LO2 in normal cells, which may be related to the down-regulation of PCNA expression.