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ATP激活P2X7受体可产生一系列的白细胞功能反应,因此P2X7受体的表达调控引起我们的兴趣。然而P2X7受体在正常人外周血单个核细胞(peripheral blood mononuclear cells,PBMC)、单核细胞中的表达调控机制尚未阐明。本文用半定量RT-PCR方法检测多种细胞因子、细菌抗原、丝裂原对P2X7受体表达的诱导作用,探索P2X7受体的诱导表达模式。结果表明,单个核细胞和单核细胞可检出P2X7受体的表达;白细胞介素2、4、6(interleukin-2、-4、-6,IL-2、IL-4、IL- 6)、肿瘤坏死因子α(tumour necrosis factor-α,TNF-α)等细胞因子和金黄色葡萄球菌Cowan Ⅰ株(Staphylococcus aureus Cowan strain I,SAC)、脂多糖(lipopolysaccharide,LPS)能上调PBMC的P2X7受体表达,而γ干扰素(interferon-γ,IFN-γ)、粒-巨噬细胞集落刺激因子(granulocyte-macrophage colony-stimulating factor,GM-CSF)、巨噬细胞集落刺激因子(macrophage colony-stimu- lating factor,M-CSF)和植物血凝素(phytohemagglutinin-M,PHA-M)等则没有作用;LPS和M-CSF可以提高单核细胞的P2X7受体表达,IFN-γ、TNF-α、GM-CSF作用较弱,但是这些因子的预处理并不能增强LPS对P2X7受体表达的诱导。炎症因子促进P2X7受体的表达,提示P2X7受体可能在对抗细菌感染的免疫反应中起一定作用,这有待于进一步研究。
ATP activates the P2X7 receptor to produce a series of leukocyte functional responses, so the expression regulation of P2X7 receptors is of interest to us. However, the mechanism of P2X7 receptor expression in normal human peripheral blood mononuclear cells (PBMCs) and monocytes has not yet been elucidated. In this paper, we used semi-quantitative RT-PCR method to detect the induction of P2X7 receptor by a variety of cytokines, bacterial antigens and mitogen, and explored the induced expression pattern of P2X7 receptor. The results showed that the expression of P2X7 receptor could be detected in mononuclear cells and monocytes; interleukin-2 (-4, -6, IL-2, IL-4, IL- , Cytokines such as tumor necrosis factor-α (TNF-α) and Staphylococcus aureus Cowan strain I (SAC) and lipopolysaccharide (LPS) up-regulated PBX P2X7 While the expression of IFN-γ, granulocyte-macrophage colony-stimulating factor (GM-CSF), macrophage colony-stimu LPS and M-CSF can enhance the expression of P2X7 receptor, monocyte chemoattractant protein-1 (IFN-γ) and TNF-α , GM-CSF had a weaker effect, but the pretreatment of these factors did not enhance the induction of P2X7 receptor expression by LPS. Inflammatory cytokines promote the expression of P2X7 receptor, suggesting that P2X7 receptor may play a role in the immune response against bacterial infection, which remains to be further studied.