【摘 要】
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Double fertilization is a key process of sexual reproduction in higher plants. The role of calcium In the activation of female sex cells through fertilization h
【机 构】
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Key Laboratory of Minlstry of Education for Plant Developmental Biology
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Double fertilization is a key process of sexual reproduction in higher plants. The role of calcium In the activation of female sex cells through fertilization has recently received a great deal of attention. The establishment of a Ca in the transduction of external stimuli, including the fusion with the sperm cell, to internal cellular processes. The present study describes the use of Fluo-3 for reporting the Ca] of the female sex cells. The results indicated that the bovine serum albumin-fusion system was superior to the polyethlene glycol.fusion system for detecting calcium fluctuations in female sex cells during fertilization. The central cell was fertilized with the sperm cell in bovine serum albumin; however, no evident calcium dynamic was detected, implying that a transient calcium rise might be a specific signal for egg cell fertilization.
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