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为了探讨运动性肠功能紊乱的原因 ,将 2 4只 SD雄性大鼠随机分为 3组 ,即对照组 (C)、运动后 30 m in组 (EX1 )、运动后 6 0 m in组 (EX2 ) ,采用化学比色法测定力竭性游泳后 30 m in和6 0 min结肠组织 MDA、Free- SH含量和 Na+ - K+ - ATPase活性。结果显示 ,运动后 30 min和 6 0m in组 MDA含量显著增加 ,Free- SH含量显著下降 ,而运动后 6 0 m in组 Na+ - K+ - ATPase活性显著下降。结果提示 ,运动源性自由基产生增加使肠组织中游离巯基被氧化 ,导致 Na+ - K+ - AT-Pase活性下降 ,可能是造成运动性肠功能紊乱的重要因素。
In order to investigate the causes of exercise-induced bowel dysfunction, 24 SD male rats were randomly divided into three groups: control group (C), 30 m in group (EX1), 60 m in group after EX EX2 ), The content of MDA, Free SH and the activity of Na + - K + - ATPase in colon tissue after exhaustive swimming for 30 min and 60 min were determined by chemical colorimetry. The results showed that the content of MDA increased significantly at 30 min and 60 min after exercise, while the content of Free SH decreased significantly. However, the activity of Na + - K + - ATPase decreased significantly at 60 min after exercise. The results suggest that the increase of exercise - derived free radicals induces the oxidation of free sulfhydryl in intestinal tissue, leading to the decrease of Na + - K + - AT-Pase activity, which may be an important factor in the development of intestinal motility disorders.