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对水稻抗病品种Jasmine85与感病品种Lemont的杂交组合构建F2 世代的单株无性系群体 ,以牙签嵌入法对 1 2 8个无性系群体进行纹枯病菌接种 ,选择极端抗感无性系构建抗感近等基因池 .用 94个均匀分布于 1 2条染色体上的多态性探针 ,与抗感近等基因池进行Southern杂交 ,从池间筛选出 3个阳性多态性分子标记 ,进而检测到 3个主效抗病QTLs(暂命名为Rh 2 ,Rh 3和Rh 7) .它们均来自抗病亲本Jasmine85 ,分别位于第 2 ,3和 7染色体上 ,各自能解释表型抗性变异的 1 4 4% ,2 6 1 %和 2 2 2 % .这些QTLs与控制株高的基因之间表现为独立遗传 ,但第 7染色体上 1个能够解释 46 1 %生育期表型变异的主效基因与Rh 7位于相同区间 .Rh 7的真实性有待进一步研究
A clonal population of F2 generation was constructed by crossing the resistant rice variety Jasmine85 with the susceptible cultivar Lemont. Chlorosis of R. solani were inoculated by the toothpick insertion method, and the extreme resistant clones were selected Anti-sense near isogenic pool.Using 94 polymorphic probes uniformly distributed on 12 chromosomes, Southern hybridization was performed with anti-sense near-isogenic pools, and 3 positive polymorphic molecular markers were screened from pools, Three QTLs for major disease resistance (tentatively named Rh 2, Rh 3 and Rh 7) were then detected, all from the resistant parent Jasmine 85, located on chromosomes 2, 3 and 7, respectively, which could explain phenotypic resistance 14.4%, 26.1% and 22.2% of the variation, respectively.These QTLs showed independent inheritance with the genes controlling the plant height, but one gene on chromosome 7 could explain the phenotypic variation at 461% The major gene is located in the same region as Rh 7. The authenticity of RH7 is for further study