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目的:探讨肝组织中Fas抗原表达和细胞凋亡的关系及其在乙型病毒性肝炎发病机理中的意义。方法:应用Fas抗原免疫组织化学染色和原位末端标记技术(TUNEL试验),原位观察84例乙型肝炎肝组织中Fas抗原表达强度和分布及凋亡细胞密度。结果:8例正常肝组织未见有Fax抗原表达,慢性重型肝炎的Fas抗原阳性表达率为100％,明显高于慢性肝炎中重度的66.7％(P<0.01);而慢性肝炎中重度的Fas阳性率则高于慢性肝炎轻度的36.7％(P<0.05)。Fas抗原阳性细胞主要位于肝组织碎屑状坏死区和小叶内灶状坏死区。慢性肝炎轻度和慢性肝炎中重度的凋亡细胞密度分别为7.96±4.61和17.90±13.16,经统计学处理具有非常显著性差异(P<0.01);重型肝炎的凋亡细胞密度为36.95±17.28,明显高于慢性肝炎轻度和慢性肝炎中重度(P均<0.01)。连续病理切片观察,凋亡细胞和Fas抗原分布有明显的同域性。结论:乙型肝炎肝组织中的Fas抗原和细胞凋亡与肝组织损伤有关,可能在乙型肝炎发病机理中起重要作用。
Objective: To investigate the relationship between Fas antigen expression and apoptosis in liver tissue and its significance in the pathogenesis of hepatitis B virus. Methods: The Fas antigen expression and distribution and the density of apoptotic cells in 84 patients with hepatitis B were observed in situ using Fas antigen immunohistochemistry and TUNEL. Results: The expression of Fax antigen in 8 cases of normal liver tissue was not seen. The positive rate of Fas antigen in chronic severe hepatitis was 100%, which was significantly higher than that in moderate and severe chronic hepatitis (66.7%, P <0.01) The positive rate was higher than the mild chronic hepatitis 36.7% (P <0.05). Fas antigen-positive cells were mainly located in the debris necrosis of liver tissue and focal lobular necrosis area. The apoptotic cell density of moderate and severe chronic hepatitis with moderate and severe hepatitis were 7.96 ± 4.61 and 17.90 ± 13.16, respectively, with statistical significance (P <0.01). The density of apoptotic cells in severe hepatitis was 36.95 ± 17.28 , Significantly higher than those of mild and chronic hepatitis with chronic hepatitis (all P <0.01). Continuous pathological sections observed, apoptotic cells and Fas antigen distribution of obvious homology. Conclusion: Fas antigen and apoptosis in liver of hepatitis B are related to liver injury, which may play an important role in the pathogenesis of hepatitis B.