目前,已有不少关于平菇单孢杂交育种的研究报道,其中大多是采用平板稀释涂布孢子悬液法分离单孢,并且依据锁状联合的有无来判断是否是单核菌丝,这种分离和检测方法很有可能会将多孢菌落误认为单孢菌落.平菇有性生殖为异宗配合,四极性类型.用上述方法获得的单核菌丝进行杂交时,就会出现种质不纯的严重问题.引起菌种退化,本实验通过一种简便的挑选单孢子的方法进行平菇品种间的杂交育种. At present, there are a lot of studies on the cross-breeding of flat mushroom Mushrooms, most of which use flat-plate dilution spore suspension method to isolate single spore, and according to the presence or absence of the lock to determine whether the mononuclear mycelium, This method of isolation and detection is likely to mistaken for multinospores as a single colony.Agricultural growth of Pleurotus ostreatus heterozygous, quadrupolar type.Using the method to obtain mononuclear mycelium hybrid, it will There is serious problem of impure germplasm, causing strain degeneration. In this experiment, crossbreeding of Pleurotus ostreatus varieties was conducted by a simple method of selecting single spore.