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研究我国北方地区莱姆病螺旋体的生物媒介—蜱的种类、带菌状况及在传播莱姆病中的作用。用布旗法和动物诱捕法在山林地区采集蜱，分类鉴定，直接荧光抗体染色法查蜱中肠带菌率，ＢＳＫ培养基分离病原体，单克隆抗体鉴定菌株。调查蜱叮咬率与人群莱姆病感染率、患病率以及蜱的季节消长与莱姆病慢性游走性红斑（ＥＣＭ）病例发生的季节性关系。在北方１０省（市、区）共采集到１００００多只蜱，分属为２科７属１１种，以全沟硬蜱为优势种，占８０％～９５％。４种蜱的中肠携带莱姆病螺旋体，全沟硬蜱的带菌率高达２０．００％～４５．００％。从４种蜱分离到９８株莱姆病螺旋体，其中９０％以上是从全沟硬蜱分离的。全沟硬蜱叮咬率高的人群，莱姆病的感染率和患病率也高。全沟硬蜱的季节消长与ＥＣＭ发生的季节性相一致。证实我国北方地区至少有４种蜱的中肠内携带莱姆病螺旋体，莱姆病螺旋体的主要生物媒介是全沟硬蜱。
To study the biological vectors of Borrelia burgdorferi in the northern part of China - the species of tick, the carrying status and the role in the transmission of Lyme disease. Ticks were collected in mountain and forest areas by cloth flag method and animal trapping method. The species of ticks were determined by direct fluorescent antibody staining method. Pathogenic bacteria were isolated by BSK medium and identified by monoclonal antibody. Investigate the seasonal relationship between tick bite rate and population Lyme disease prevalence, prevalence, and the seasonal fluctuation of ticks and the occurrence of chronic migratory erythema (ECM) in Lyme disease. In the northern 10 provinces (cities, districts) collected more than 10,000 ticks, belonging to 2 families and 7 genera and 11 species, all Ixodes dominants, accounting for 80% to 95%. The four species of ticks carried Lyme disease in the midgut, and the rate of carrying Ixodes was up to 20.00% -45.00%. 98 strains of Borrelia burgdorferi were isolated from 4 species of ticks, of which over 90% were isolated from Ixodes. The population with high bite rate of full-fledged ticks also has a high prevalence and prevalence of Lyme disease. The seasonal dynamics of Ixodes latipes coincide with the seasonal occurrence of ECM. Confirmed that at least 4 kinds of ticks in the northern region of China carry Lyme disease in the midgut, Lyme disease is the main biological medium is Ixodes ditch Ixodes.