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目的研究模拟微重力条件对拟南芥生长过程中生理、生化特性以及亚显微结构的影响。方法通过回转仪模拟微重力环境,利用激光共聚焦扫描显微镜和Ca2+荧光探针对生长7 d的拟南芥幼苗进行细胞微管骨架变化、Ca2+分布以及相关酶活力进行研究测定。结果在模拟微重力条件下拟南芥植株生长慢于对照;拟南芥叶片细胞的微管骨架有序性降低,微管数量显著增加,其排列模式呈现多样化,叶绿体含量有所减少;根尖Ca2+分布趋向均匀化,并且Ca2+向叶片组织的转运增加;过氧化物酶、过氧化氢酶活力以及抗坏血酸含量显著增加,超氧化物歧化酶活力有所增加,匀浆蛋白含量有所减少。结论模拟微重力环境对拟南芥的生物特性有显著影响,拟南芥幼苗应激反应明显。
Objective To study the effects of simulated microgravity on the physiological and biochemical characteristics and the sub-microstructure of Arabidopsis thaliana. Methods Cytoskeletal changes, Ca2 + distribution and related enzyme activities of Arabidopsis thaliana seedlings grown for 7 d were studied by laser confocal scanning microscopy and Ca2 + fluorescence probe. Results The Arabidopsis thaliana grew slower than the control under simulated microgravity conditions. The microtubule structure of Arabidopsis thaliana decreased in sequence and the number of microtubules was significantly increased. The arrangement pattern of the Arabidopsis thaliana was diversified and the content of chloroplast decreased. The distribution of Ca2 + in tip was homogenized and the Ca2 + transport to leaf tissue was increased. The activities of peroxidase, catalase and ascorbic acid were significantly increased, the activity of superoxide dismutase increased and the content of homogenate protein decreased. Conclusion Simulated microgravity environment had a significant effect on the biological characteristics of Arabidopsis thaliana, and the stress response of Arabidopsis thaliana seedlings was obvious.