生化分析证实大骨节病患儿红细胞低硒,脂质组成异常,膜的脆性增加,抗过氧化能力降低,存在着“膜缺陷”。扫描电镜观察患儿红细胞,见有较多棘细胞和口状细胞形成,认为与红细胞膜缺陷有关。为进一步探讨膜的变异,作者用透射电镜方法,对大骨节病患儿及对照儿童的红细胞作对照观察。取静脉血,肝素抗凝,生理盐水洗涤离心,反复三次,再分别作常规电镜标本及冷冻复型标本。常规电镜标本见红细胞表面有明确界限包绕,内部充满电子密度高的均质物质,红细胞断面外形各异。在高倍视野下,对照儿童红细胞膜可见暗·明·暗三层, Biochemical analysis confirmed that children with Kashin-Beck disease erythrocyte selenium, lipid composition abnormalities, increased membrane fragility, reduced anti-oxidative capacity, there is a “membrane defect.” Scanning electron microscopy of children with red blood cells, see more spine cells and the formation of oral cells, that the red cell membrane defects. To further explore the membrane variation, the author using transmission electron microscopy methods for children with children with Kashin-Beck disease and control children’s red blood cells as a control. Take venous blood, heparin anticoagulation, normal saline washing centrifuge, repeated three times, and then for conventional electron microscopy specimens and frozen complex type specimens. Conventional electron microscopy see the red blood cell surface has a clear boundary surrounded by internal homogeneous electron density of homogeneous material, red blood cell profiles of different shapes. In high power, the control children’s red blood cell membrane can be seen dark, bright and dark three,