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目的探讨Ｐ－糖蛋白（Ｐ－ｇｌｙｃｏｐｒｏｔｅｉｎ，Ｐ－ｇｐ）在初治老年人急性髓细胞白血病（ＡＭＬ）中的表达特点及其预后意义。方法采用流式细胞术（ｆｌｏｗｃｙｔｏｍｅｔｒｙ，ＦＣＭ）及抗Ｐ－ｇｐ细胞膜外区域单克隆抗体ＵＩＣ２检测了３７例初治老年人ＡＭＬ白血病细胞Ｐ－ｇｐ表达。结果１６例（４３％）患者Ｐ－ｇｐ阳性（Ｐ－ｇｐ＋），Ｐ－ｇｐ与干细胞或祖细胞抗原ＣＤ３４表达显著相关，与其它免疫表型无关。在可评估疗效的３２例中，Ｐ－ｇｐ＋１５例中的４例（２７％）获得完全缓解（ＣＲ），缓解率显著低于Ｐ－ｇｐ阴性（Ｐ－ｇｐ－）１７例中的１１例（６５％）（Ｐ＜０．０５）。ＣＤ３４阳性（ＣＤ３４＋）１４例中ＣＲ３例（２１％），也显著低于ＣＤ３４阴性（ＣＤ３４－）者１８例中１２例（６７％）（Ｐ＜０．０５）。Ｐ－ｇｐ－及ＣＤ３４－１３例中１１例（８５％）获得ＣＲ。结论Ｐ－ｇｐ阳性及ＣＤ３４阳性是老年人ＡＭＬ预后差的重要指标
Objective To investigate the expression characteristics and prognostic significance of P-glycoprotein (P-gp) in elderly patients with acute myeloid leukemia (AML). Methods The expression of P-gp in 37 newly diagnosed AML leukemia cells was detected by flow cytometry (FCM) and anti-P-gp extracellular membrane monoclonal antibody UIC2. Results P-gp positive (P-gp+) was detected in 16 patients (43%). P-gp was significantly associated with the expression of stem or progenitor cell antigen CD34, but not with other immunophenotypes. Of the 32 patients with assessable efficacy, 4 (27%) of P-gp+15 patients achieved complete remission (CR), with a significantly lower remission rate than 11 of 17 P-gp-negative (P-gp-) patients. 65%) (P<0.05). CD34-positive (CD34+) was found in 14 cases of CR3 (21%) and also significantly lower than CD34-negative (CD34-) of 12 cases (67%) in 18 cases (P<0.05). CR was obtained in 11 cases (85%) of P-gp- and CD34-13 cases. Conclusion P-gp positive and CD34 positive are important indicators of poor prognosis of elderly AML