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目的:通过观察大脑中动脉闭塞(MCAO)大鼠血浆TAT、F1+2、D-二聚体、v WF含量的动态变化,探讨MCAO大鼠缺血后血栓生物标记物的变化规律,为药物研究确定最佳观察时间点。方法:雄性SD大鼠56只,随机分为:假手术组,缺血后不同时相组(1 h、6 h、12 h、24 h、48 h、72 h),每组8只。线栓法制造大脑中动脉闭塞大鼠模型,用ELISA法检测大鼠血浆TAT、F1+2、D-二聚体、v WF的水平。结果:脑缺血后1h TAT的水平即上升且于24h达峰值;F1+2、D-二聚体的水平在缺血后1 h骤然升高并于6h达峰值;而v WF在缺血后1h即骤然升高达峰值,然后维持在稳定的较高水平。结论:MCAO大鼠在急性脑缺血后血浆TAT、D-二聚体、v WF、F1+2水平均升高,表现出不同的时程规律,它们的峰值均集中在缺血后6 h或24 h,提示脑缺血的早期即存在有凝血纤溶系统紊乱、血管内皮细胞损伤,缺血后24 h可作为药物研究的最佳观察时间点。
OBJECTIVE: To observe the changes of plasma TAT, F1 + 2, D-dimer and v WF contents in rats with middle cerebral artery occlusion (MCAO) to investigate the changes of thrombotic biomarkers after ischemia in MCAO rats. Study to determine the best time to observe. Methods: Fifty-six male Sprague-Dawley rats were randomly divided into sham-operation group, ischemia group and untreated group (1 h, 6 h, 12 h, 24 h, 48 h, 72 h). The rat model of middle cerebral artery occlusion was established by the suture method. The levels of plasma TAT, F1 + 2, D-dimer and v WF were detected by ELISA. Results: The level of TAT increased 1 hour after cerebral ischemia and peaked at 24h. The levels of F1 + 2 and D-dimer increased suddenly 1 h after ischemia and peaked at 6 h. However, After 1h suddenly increased peak, and then maintained at a stable high level. CONCLUSION: The levels of TAT, D-dimer, v WF and F1 + 2 in MCAO rats after acute cerebral ischemia all show different time-course rules, and their peak values are all concentrated at 6 h after ischemia Or 24 h, suggesting that the early cerebral ischemia that there is coagulation and fibrinolysis system disorders, vascular endothelial cell injury, 24 h after ischemia can be used as the best time to observe the drug.