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目的探讨白细胞介素12B(IL-12B)基因1188A/C位点多态性与山东汉族人群慢性阻塞性肺疾病(COPD)易感性的关系。方法采用PCR-RFLP法对324例COPD患者和389例健康人IL-12B基因1188A/C位点多态性进行分型,对等位基因及基因型分布进行统计学分析。结果IL-12B基因1188A/C多态位点AA、AC、CC基因型频率在病例组中分别为0.333 30、.478 4和0.188 3,在对照组中分别为0.357 30、.475 6和0.167 1,A和C等位基因型的频率在病例组中分别为0.572 5和0.427 5,在对照组分别为0.595 1和0.404 9,该位点基因型和等位基因型分布在病例组与对照组P值分别为0.689,0.389,差异均无统计学意义。结论IL-12B基因1188A/C位点多态性与山东汉族人群COPD发生无明显相关性。
Objective To investigate the association of 1188A / C polymorphism of interleukin 12B (IL-12B) gene with susceptibility to chronic obstructive pulmonary disease (COPD) in Shandong Han population. Methods Polymorphisms of 1188A / C polymorphism in IL-12B gene of 324 patients with COPD and 389 healthy subjects were genotyped by PCR-RFLP, and the distribution of alleles and genotypes were statistically analyzed. Results The frequency of AA, AC and CC genotypes in 1188A / C polymorphism site of IL-12B gene were 0.333 30, 4.78 4 and 0.188 3 respectively in the case group and 0.357 30, .475 6 and 0.167 respectively in the control group 1, A and C allele frequencies were 0.572 5 and 0.427 5 in the case group and 0.595 1 and 0.404 9 in the control group, respectively. The genotypes and alleles of this locus were distributed between the case group and the control Group P values were 0.689,0.389, the difference was not statistically significant. Conclusion There was no significant correlation between IL-12B gene 1188A / C polymorphism and COPD in Shandong Han population.