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经接合转移将luxAB基因插入快生型大豆根瘤菌 (Rhizobiumfredii)染色体 ,取可高效结瘤固氮的R .frediilux3菌株用于分子生态学研究 .在不同基模势下 ,研究了lux3在土壤中的存活 ,基模势为 - 30和 - 750kPa时 ,lux3在灭菌土中的生存能力明显 (P<0 .0 5)高于未灭菌土 ,当基模势为 - 1 50 0kPa时 ,土壤是否灭菌对lux3的存活影响没有差异 .不同基模势对lux3活性影响显著 ,在 - 1 50 0kPa的未灭菌土中 ,随着饥饿时间延长 ,可恢复活性的细菌数量显著下降 .同时比较了测定微生物活性的 3种方法 (即生物发光、脱氢酶活性和微生物呼吸 ) ,发光值变化与活细胞密度变化一致 ,PLf值 (即潜在发光 ,Potentialluminescence)反映了可恢复活性的饥饿种群密度 .luxAB基因是一种比较简便而实用的标记
The luxAB gene was inserted into the chromosome of Rhizobium fredii through conjugation and transfer, and the Rfrediilux3 strain, which can efficiently nodulate and fix nitrogen, was used in the molecular ecology research.Under the different mode potentials, lux3 was studied in the soil Survival and luxilities of lux3 in sterilized soil were significantly higher (P <0. 05) than those of non-sterilized soil at base pressures of -30 and -75kPa, respectively. When the base-mode potential was -150kPa, There was no difference in the effect of sterilization on lux3 survival.Effect of different base modes on lux3 activity was significant, and the number of bacteria that could be restored to active state decreased significantly with the increase of starvation time in -150 0 kPa unsterilized soil. Three methods for measuring the activity of microorganisms (ie, bioluminescence, dehydrogenase activity and respiration of microorganisms) showed that the changes of luminescence values are consistent with the changes of living cell densities. The PLf value (ie, potential luminescence) reflects the recoverable activity of starved population density The luxAB gene is a simpler and more practical marker