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目的:研究了谷胱甘肽(还原型,glutathione,GSH)在咖啡酸(Caffeic acid,CFA)修饰碳糊电极(CFA/CPE)上的电催化氧化行为和电化学分析方法。方法:循环伏安法(CV),计时电流法(CA)和线性扫描伏安法(LSV)。结果:GSH在碳糊电极(CPE)上的直接电化学氧化过程十分迟缓,CFA/CPE对GSH电化学氧化具有良好的催化作用。同时测定了GSH在CFA/CPE上的电极过程动力学参数,用LSV法测得催化氧化峰电流与GSH在5.0×10-5～1.0×10-3 mol.L-1浓度范围内呈良好线性关系,线性回归方程为Ipa(μA)=2.003c(10-3 mol.L-1)+3.448,r=0.9989,检出限为4.0×10-5 mol.L-1。结论:CFA/CPE对GSH电化学氧化具有良好的催化作用,该方法可用于市售还原型谷胱甘肽药物含量的电化学定量测定。
OBJECTIVE: To study the electrocatalytic oxidation behavior of glutathione (GSH) on CFA / CPE electrode and its electrochemical analysis. Methods: Cyclic voltammetry (CV), chronoamperometry (CA) and linear sweep voltammetry (LSV) were performed. Results: The direct electrochemical oxidation of GSH on the carbon paste electrode (CPE) was very slow. CFA / CPE had a good catalytic effect on the electrochemical oxidation of GSH. The kinetic parameters of GSH on CFA / CPE electrode were also determined. The catalytic oxidation peak current and GSH were linear with the concentration of 5.0 × 10-5 ~ 1.0 × 10-3 mol.L-1 The linear regression equation was Ipa (μA) = 2.003c (10-3 mol.L-1) + 3.448, r = 0.9989 with a detection limit of 4.0 × 10-5 mol·L-1. CONCLUSION: CFA / CPE has a good catalytic effect on the electrochemical oxidation of GSH. This method can be used for the electrochemical quantitative determination of GSH.