利用荧光光谱和同步荧光光谱研究了不同温度下苯胺蓝黑与人血清白蛋白相互作用时的荧光猝灭及构象的变化情况。实验结果表明,苯胺蓝黑与人血清白蛋白之间可以发生相互作用,而且有较强的结合。同步荧光光谱研究了人血清白蛋白与苯胺蓝黑的相互作用中人血清白蛋白构象的变化,结果显示二者结合改变了蛋白质的微环境。热力学参数说明小分子与蛋白质的作用以疏水作用为主。 Fluorescence quenching and conformational changes of the interaction between aniline blue and human serum albumin at different temperatures were studied by fluorescence spectroscopy and synchronous fluorescence spectroscopy. The experimental results show that the interaction between aniline blue and human serum albumin, but also a strong combination. Synchronous fluorescence spectra of human serum albumin and aniline blue interaction of human serum albumin conformation changes, the results show that the combination of the two changed the protein microenvironment. Thermodynamic parameters that small molecules and the role of the protein to the role of hydrophobic.