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目的分析活体正电子发射断层扫描术(PET)测试兔眼内σ_1受体的显像情况。设计实验研究。研究对象健康家兔5只和大鼠20只。方法应用放射性标记物[~(11)C]SA4503对家兔行PET的基础测定、预处理测定和置换测定及体外放射自显影术(ex vivo ARG)检查;对20只大鼠行体外放射自显影术检查,通过与σ_1受体结合测定眼球内组织的放射能摄取率。主要指标眼内σ_1受体在PET显像中的分布情况。结果PET下兔眼内虹膜睫状体与视网膜可以显像,并且通过氟呱啶醇的预处理和置换实验,证实PET图像反映了放射性标记物的特异性结合。大鼠眼内可以发现[~(11)C]SA4503大量聚集在虹膜睫状体和视网膜组织。载体负荷实验则显示[~(11)C]SA4503的受体特异性结合约占脑内总结合率的75%。σ_1受体存在于视网膜至上丘的神经轴突。体外放射自显影术证实兔眼和鼠眼内虹膜睫状体和视网膜组织存在高密度的σ_1受体。结论虹膜睫状体和视网膜中均富含σ_1受体, PET可用于活体眼内神经受体分布的研究。
Objective To analyze the positron emission tomography (PET) test of intraocular sigma 1 receptor imaging. Design experiment research. Five healthy rabbits and 20 rats were studied. Methods The basal, pretreatment, and ex vivo ARG measurements of radioactive markers [~ (11) C] SA4503 were performed in rabbits. In vitro radioactivity Developmental imaging examines the intraocular tissue radioactivity uptake by binding to the sigma 1 receptor. The main indicators intraocular sigma 1 receptors in PET imaging distribution. Results Intraocular iris and ciliary body can be visualized in retina of PET under PET, and the pretreatment and displacement experiments with Fidagnidol confirmed PET images reflect the specific binding of radioactive labels. In the rat eye, [~ (11) C] SA4503 was found to accumulate in large amounts in the iris ciliary body and the retina. The carrier loading experiments showed that the receptor specific binding of [~ (11) C] SA4503 accounted for about 75% of the total brain binding rate. The sigma-1 receptor is present in the axon of the optic nerve in the retina to the superior colliculus. In vitro autoradiography confirmed the presence of a high density of sigma 1 receptors in the iris and ciliary body of retina and rabbit eye. Conclusion Both the iris ciliary body and the retina are rich in sigma-1 receptor. PET can be used to study the distribution of neural receptors in living eyes.