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人体外受精(in vitro fertilization,IVF)技术当前面临进退两难,因为采用氯菧酚胺或人绝经期促性腺素等诱导排卵剂时,不可能准确地控制滤泡数目。作者认为对多余的卵子及胚胎应用于研究,这将可改进IVF操作,减少胚胎缺陷及增加IVF的应用及有关技术。胚胎冷冻的建立 1972年首先报告哺乳动物冷冻胚胎成功。用二甲亚砜(DMSO)作小鼠8-细胞胚胎冷冻保护后,使缓慢冷至-196℃,可获得存活小鼠。以后在兔、羊、山羊及牛的胚胎用改良上法亦获得成功。这些动物实验表明,胚胎缓慢降温至-80℃需缓慢解冻,而转移胚胎到液氮-30°至-40℃时,则需要迅速
In vitro fertilization (IVF) technology is currently faced with dilemmas because it is impossible to control the number of follicles accurately when ovulation induction agents such as clopidogrel or human menopausal gonadotropin are used. The authors believe that the application of excess eggs and embryos to research, which will improve IVF operation, reduce embryonic defects and increase the application of IVF and related technologies. Establishment of embryo freezing First reported in mammalian frozen embryos in 1972 was successful. After the mouse 8-cell embryos were cryoprotected with dimethyl sulfoxide (DMSO), the mice were allowed to cool slowly to -196 ° C. Later on, the improved method on embryos of rabbits, sheep, goats and cattle also succeeded. These animal experiments show that embryos slowly thawing to -80 ℃ need to be slowly thawed, and the transfer of embryos to liquid nitrogen -30 ° to -40 ℃, you need to quickly