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本文介绍了一种适于大量制备钙调素（ＣａＭ）的简易实用方法。我们用该方法从２．５ｋｇ猪脑组织中一次纯化了１５０ｍｇＣａＭ。经鉴定，所提ＣａＭ的纯度在ＳＤＳＰＡＧＥ中呈单一区带，分子量为１９ｋＤ（在１ｍｍｏｌ／ＬＥＧＴＡ的条件下），等电点为４．３８，并对ＰＤＥ有明显的激活作用。该作用可被特异性抑制剂ＴＦＰ抑制。
This article presents a simple and practical method for preparing large quantities of calmodulin (CaM). We used this method to purify 150 mg CaM from 2.5 kg porcine brain tissue once. The purity of CaM was identified as a single band in SDSPAGE with a molecular weight of 19kD (under the condition of 1mmol / L EGTA) and the isoelectric point was 4.38, which markedly activated PDE. This effect can be inhibited by the specific inhibitor TFP.