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为了解甘蔗组织培养过程中同工酶的变化情况,我们对桂糖11号、桂糖2号两个组织培养材料的酯酶和过氧化物酶同工酶进行了分析,以探讨同工酶变化和细胞脱分化、分化的关系。材料和方法材料:桂糖11号、桂糖2号两个品种,以尾梢幼嫩部分为培养材料,分别取外植体(培养8天)、愈伤组织、胚性细胞团、小苗(长1寸)、大苗(长3寸)各期材料。样品制备:取各期材料200毫克,加入
In order to understand the changes of isozymes during the tissue culture of sugarcane, we analyzed the esterase and peroxidase isoenzymes of two tissue culture materials, Guitang 11 and Guigang 2, Changes and dedifferentiation of cells, the relationship between differentiation. MATERIALS AND METHODS MATERIALS: Two varieties of Guitang No. 11 and Guitang No. 2 were taken from the young part of the tail tip as the culture material, and the explants (cultured for 8 days), callus, embryogenic cell mass, seedling ( 1 inch long), big seedlings (3 inches long) of various materials. Sample Preparation: Take 200 mg of each material, add