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为了研究乳癌相关肽 (pS2 ,为三叶肽家族一员 )、肠三叶因子 (intestinaltrefoilfactor,ITF ;为三叶肽家族一员 )在水浸束缚应激(WRS)大鼠胃粘膜基因表达变化 ,探讨其在应激胃粘膜损伤的早期修复作用。采用单次水浸束缚应激制作模型 ,动态监测胃粘膜血流量(GMBF) ,大体及光镜下观察粘膜损伤程度(UI)及组织学变化 ,逆转录 多聚酶链反应 (RT PCR)检测pS2 、ITF基因表达变化 ,免疫组化染色进一步证实其表达。结果显示 :应激造成胃粘膜广泛损伤 ,但损伤指数在 2、4、8h逐渐减小 ,至 8h降为 6 4 9% ,GMBF逐渐恢复 ,至8h上升为正常的 89 8% ,ITF基因表达逐渐增强(0 0 2 2±0 0 0 1vs0 177±0 0 10 ,P <0 0 1) ,免疫组化染色计分为 (0 134± 0 0 0 1vs0 2 5 3±0 0 1,P <0 0 1) ;pS2 基因表达逐渐增强 (0 5 1± 0 14vs 0 77± 0 11,P <0 0 1) ,免疫组化染色计分为 (0 95± 0 11vs 1 4 1± 0 0 4 ,P <0 0 1)。提示pS2 、ITF可能均参与了胃粘膜早期重建
Gastric mucosa gene expression changes in a water-immersion restraint stress (WRS) rat model in order to study breast cancer-associated peptide (pS2, a member of the trefoil peptide family), intestinal trefoil factor (ITF; a member of the trefoil peptide family) , To explore its early repair of gastric mucosal injury in stress. Gastric mucosal blood flow (GMBF) was dynamically monitored by a single immersion restraint stress model. The degree of mucosal injury (UI) and histological changes were observed under light and light microscope. The expression of pS2 was detected by reverse transcription polymerase chain reaction (RT PCR) ITF gene expression changes, immunohistochemical staining further confirmed its expression. The results showed that the stress caused a wide range of gastric mucosal injury, but the injury index gradually decreased from 2 to 4, 8 to 6 4 9%, and GMBF recovered gradually to 89 8% after 8 h. ITF gene expression (0 0 2 2 ± 0 0 0 1vs0 177 ± 0 0 10, P 0 01). The score of immunohistochemical staining was (0 134 ± 0 0 0 1vs0 2 53 ± 0 0 1, P < The expression of pS2 gene was gradually increased (0 5 1 ± 0 14 vs 0 77 ± 0 11, P 0 01). The score of immunohistochemical staining was (0 95 ± 0 11 vs 1 4 1 ± 0 0 4 , P <0 0 1). Tip pS2, ITF may be involved in the early reconstruction of gastric mucosa