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本文应用增殖细胞核抗原（PCvA）免疫组织化学方法和核仁组成区相关嗜银蛋白（AeNORS）嗜报染色方法，研究了膀胱移行细胞癌增殖活性。结果发现该癌的PCNA指数分别为：Ⅰ级11．7％（n＝17．x±1．93），Ⅱ级21．95％（n＝17．x±2．11），Ⅱ级48．63％（n＝17，x±1．85），三组比较，经H检验分析差异有显著性（p＜0．005），三组间两两比较均有显著性差异（p＜0．01）；膀胱移行细胞癌每核AgNORS平均数分别为：Ⅰ级4．56（n＝17，x±0．06），Ⅱ级6．63（n＝17，x±0．09）．Ⅲ级9．73（n＝17，x±0．34）,三组比较，经H检验分析差异有显著性（p＜0．005），三组间两两比较均有显著性差异（p＜0．01）；51个膀胱癌标本的每核AgNORS平均数和PCNA指数作相关性分析，两者有很好的相关性（r＝0．646，p＜0．005）。结果证明PCNA指数和每核AgNORS平均数与膀胱癌的组织学分级有关，肿瘤的分化程度越低，PCNA指数和每核AgNORS平均数越高。提示PCNA和AgNORS染色可提高膀胱癌组织学分级的准确性和可重复性，对临床制订合理的治疗方案和评估病人的预后有一定的指导意义。
In this study, proliferating cell nuclear antigen (PCvA) immunohistochemistry and nucleolar organizer region associated with silver staining (AeNORS) addictive staining method was used to study the proliferation of bladder transitional cell carcinoma. The results showed that the PCNA index of the cancer was 11.7% for grade Ⅰ (n = 17.x ± 1.93), 21.95% for grade Ⅱ (n = 17.x ± 2.11) and 48 for grade Ⅱ. 63% (n = 17, x ± 1.85). There was significant difference between the three groups (P <0.005) by H test (p <0.005). 01). The average number of AgNORS per bladder transitional cell carcinoma was grade Ⅰ 4.56 (n = 17, x ± 0.06) and grade Ⅱ 6.63 (n = 17, x ± 0.09). Ⅲ grade 9.73 (n = 17, x ± 0.34). There was significant difference between the three groups (p <0.005) by H test (p <0.005), p < <0.01). There was a good correlation between the average AgNORS per core of 51 bladder cancer samples and PCNA index (r = 0.646, p <0.005). The results show that the PCNA index and the average number of AgNORS per core and bladder cancer histological grade related to the lower the degree of tumor differentiation, PCNA index and the average number of AgNORS per core higher. It is suggested that the staining of PCNA and AgNORS can improve the accuracy and repeatability of histological grading of bladder cancer, which is of guiding significance in establishing a reasonable treatment plan and assessing the prognosis of patients.