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目的 :研究p16基因的纯合性缺失、突变及表达异常与卵巢癌的发生是否存在相关关系。方法 :采用PCR SSCP检测卵巢癌 p16基因的纯合性缺失和突变 ;采用RP PCR定性及半定量分析p16基因 ;采用免疫组化技术检测 p16蛋白。 结果 :3 2例卵巢癌中检测到 1例外显子 1突变 ,未检测到 p16基因的纯合性缺失 ;RT PCR分析卵巢癌中p16基因mRNA均有表达 ,半定量分析卵巢癌与正常卵巢组织中 p16基因mRNA表达水平无差别 ;免疫组化检测 9例卵巢癌中的 p16蛋白为阴性 (2 8.2 % )。 结论 :卵巢癌的发生与 p16基因的纯合性缺失和突变无相关关系 ,与p16基因异常表达有相关关系
OBJECTIVE: To study whether the homozygous deletion, mutation and abnormal expression of p16 gene are correlated with the occurrence of ovarian cancer. Methods: The homozygous deletion and mutation of p16 gene in ovarian cancer were detected by PCR SSCP. The p16 gene was analyzed qualitatively and semiquantitatively by RP-PCR. The p16 protein was detected by immunohistochemistry. Results: One case of exon 1 mutation was detected in 32 cases of ovarian cancer and no homozygous deletion of p16 gene was detected. The mRNA expression of p16 gene in ovarian cancer was detected by RT-PCR. Semi-quantitative analysis of ovarian cancer and normal ovarian tissue There was no difference in the expression of p16 mRNA among all the groups. Immunohistochemistry showed that p16 protein was negative in 9 cases of ovarian cancer (2 8.2%). Conclusion: There is no correlation between the occurrence of ovarian cancer and the homozygous deletion and mutation of p16 gene, and the abnormal expression of p16 gene