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目的:制备丹酚酸B壳聚糖纳米粒,并考察对大鼠心脏缺血/再灌注损伤的保护作用。方法:采用离子凝胶化法制备丹酚酸B壳聚糖纳米粒,并以包封率(EE%)和粒径分布(nm)为评价指标,对丹酚酸B壳聚糖纳米粒处方进行优化;采用Malvern粒度仪测定纳米粒的粒径分布和Zeta电位,透射电镜考察其形态;并考察丹酚酸B壳聚糖纳米粒的体外释药行为;考察丹酚酸B壳聚糖纳米粒对大鼠心脏缺血/再灌注损伤的保护作用。结果:丹酚酸B壳聚糖纳米粒的包封率为85.8%±3.1%;粒径为(166.1±42.4)nm,Pd I为(0.189±0.032),Zeta电位为(+24.9±4.5)m V;透射电镜显示丹酚酸B壳聚糖纳米粒粒径均一,成球状;纳米粒在24 h内平稳缓慢释药;丹酚酸B壳聚糖纳米粒可以增加大鼠心脏缺血/再灌注损伤的保护作用。结论:丹酚酸B壳聚糖纳米粒对大鼠心脏缺血/再灌注损伤具有良好的保护作用。
OBJECTIVE: To prepare salvianolic acid B chitosan nanoparticles and study its protective effect on myocardial ischemia / reperfusion injury in rats. Methods: The salvianolic acid B chitosan nanoparticles were prepared by ion-gel method. The encapsulation efficiency (EE%) and particle size distribution (nm) The particle size distribution and Zeta potential of nanoparticles were measured by Malvern particle size analyzer. The morphologies of the nanoparticles were investigated by transmission electron microscopy. The in vitro drug release behavior of salvianolic acid B chitosan nanoparticles was investigated. Protective effects of granule on myocardial ischemia / reperfusion injury in rats. Results: The encapsulation efficiency of salvianolic acid B chitosan nanoparticles was 85.8% ± 3.1%, the particle size was (166.1 ± 42.4) nm, the Pd I was (0.189 ± 0.032) and the Zeta potential was (+ 24.9 ± 4.5) m V. The transmission electron microscopy showed that salvianolic acid B chitosan nanoparticles were uniform in size and spherical in shape. The nanoparticles released slowly and smoothly within 24 h. The salvianolic acid B chitosan nanoparticles could increase the expression of myocardial ischemia / Reperfusion injury protection. Conclusion: Salvianolic acid B chitosan nanoparticles have a good protective effect on myocardial ischemia / reperfusion injury in rats.