鉴定那些涉及细胞周期调控的蛋白质,有助于我们理解细胞生长和转化的机制。前不久,作者利用双维凝胶电泳等技术,发现了一种被称为周期素(cyclin)的细胞核蛋白其分子量为36KDa。周期素在非分裂正常细胞合成量很少,而在处于增殖状态的细胞和转化细胞中其合成量则大为增加。在人、小鼠、仓鼠和鸟类都发现了周期素。免疫萤光和放射自显影研究表明:周期素在细胞周期的 S 期 Identifying those proteins involved in cell cycle regulation helps us understand the mechanisms of cell growth and transformation. Not long ago, the authors used techniques such as two-dimensional gel electrophoresis and found that a nuclear protein called cyclin has a molecular weight of 36 kDa. Cyclin is synthesized in non-dividing normal cells in very small quantities, whereas the amount of synthesis is significantly increased in proliferating and transformed cells. Cyclins are found in humans, mice, hamsters and birds. Immunofluorescence and autoradiography studies show that cyclin is in the S phase of the cell cycle