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以丙烯酰胺为功能单体、N,N’-亚甲基双丙烯酰胺为交联剂、溶菌酶为模板蛋白,用反相悬浮聚合法制备了溶菌酶分子印迹微球。所得微球外观圆整,平均粒径为34.6μm,ζ电位为-33.5 mV。考察了溶菌酶印迹微球在水和生理盐水两种介质中对溶菌酶、核糖核酸酶A或牛血清白蛋白的吸附量,以及微球在生理盐水中对两种蛋白质混合溶液中溶菌酶的特异性吸附能力。结果表明,微球对溶菌酶的吸附在40 min内达到平衡。无论在单一蛋白质还是有其它蛋白质干扰的竞争环境中,印迹微球对模板蛋白溶菌酶都表现出更强的吸附能力。在生生理盐水介质中,由于降低了非特异性吸附效应,印迹微球对模板蛋白的选择性吸附更显著。
Lysozyme molecularly imprinted microspheres were prepared by reversed-phase suspension polymerization using acrylamide as the functional monomer, N, N’-methylenebisacrylamide as the crosslinking agent and lysozyme as the template protein. The resulting microspheres were rounded, with an average particle size of 34.6 μm and a zeta potential of -33.5 mV. The adsorption amount of lysozyme, ribonuclease A or bovine serum albumin of lysozyme imprinted microspheres in water and saline medium was investigated, as well as the adsorption of lysozyme in the mixed solution of microspheres in physiological saline Specific adsorption capacity. The results showed that the adsorption of lysozyme microspheres reached equilibrium within 40 min. The imprinted microspheres showed stronger adsorption capacity for the template protein lysozyme in the competitive environment of single protein or other protein interference. In physiological saline solution, the selective adsorption of template proteins by the imprinted microspheres was more pronounced due to the reduced nonspecific adsorption effect.